| Method |
Type of Peptide or Protein to be Attached |
| EDC Attachment |
Peptides and linkers that contain carboxylic acids such as aspartic acid, glutamic acid and end terminus carboxylic acids |
| AMAS/Traut's Reagent |
Peptides and proteins that contain free primary amines such as unblocked lysine |
| AMAS/DTT |
Antibodies and peptides that have cysteine bridges that can be used for conjugation |
| HBTU/HOBt Coupling |
Carboxyl-substituted peptides, such as those containing aspartic and glutamic amino acids, that do not contain primary amines and can be activated in DMF |
| Covalent Attachment of Activated Carboxylic Acids |
Activated carboxyl-substituted peptides or proteins such as N-hydroxysuccinimide esters |